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METHOD DETAILS Sample preparation FOXO4 FHD (95-195) was subcloned into the pET His6 Tobacco Etch Virus (TEV) LIC cloning vector (2B-T, obtained from Scott Gradia, Addgene Plasmid #29666) and transformed into BL21 (DE3) cells

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ERK1/2 activation in endothelial cells with upregulation of c-Fos, c-Jun, and Egr-1, regulating cell proliferation and vascular tube formation in vitro

In the STEP UP clinical trial, the 7.2-mg dose demonstrated a mean weight loss of 20.7% at 72 weeks, compared with a reduction of 17.5% with the previously approved semaglutide (Wegovy) injection (2.4 mg) and 2.4% with placebo