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The biomechanical and biochemical changes would together disrupt cartilage homeostasis and contribute to the pathogenesis of OA, which leads to joint space narrowing, painful cartilage destruction, and loss of function

These intramuscular injections, usually containing cyanocobalamin or methylcobalamin, are administered weekly or monthly as prescribed by a healthcare provider

The three libraries were generated using error-prone PCR as described above, using the following conditions to produce varying levels of mutagenesis: Library 1: 2 M 8-oxo-dGTP, 0.5 M dPTP, 20 PCR cycles Library 2: 2 M 8-oxo-dGTP, 1 M dPTP, 20 PCR cycles Library 3: 2 M 8-oxo-dGTP, 2 M dPTP, 20 PCR cycles The library sizes (approximated by number of transformants as described above) were 6 10 7 for each library

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