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Groups of ten size-matched islets were incubated at 37 C in HEPES-buffered Krebs-Ringer bicarbonate buffer (HKRB) composed of (in mM) 4.7 KCl, 1.2 KH 2 PO 4 , 129 NaCl, 5 NaHCO 3 , 1.2 MgSO 4 , 1.8 CaCl 2 , and 10 HEPES, with pH adjusted at 7.4 using NaOH supplemented with 2.8 mM glucose, followed by test incubation for 1 h with 2.8 or 11.2 mM glucose in the absence or presence of d-allulose (20 mM) or d-mannitol (20 mM)
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Peptide Vials (GHK-Cu, 50 mg each): 5 days/week (1.0-2.0 mg/day): 8 weeks (~50 mg total): 1 vial 12 weeks (~90 mg total): 2 vials 16 weeks (~130 mg total): 3 vials 3 times/week (2 mg each): 8 weeks (~48 mg total): 1 vial 12 weeks (~72 mg total): 2 vials 16 weeks (~96 mg total): 2 vials Insulin Syringes (U-100, 29-31 gauge): 5 days/week: Per week: 5 syringes 8 weeks: 40 syringes 12 weeks: 60 syringes 16 weeks: 80 syringes 3 times/week: Per week: 3 syringes 8 weeks: 24 syringes 12 weeks: 36 syringes 16 weeks: 48 syringes Sterile or Bacteriostatic Water (10 mL bottles): Use 3.0 mL per vial for the displayed calculation
Drugs That Can Harm an Unborn Baby (Teratogenic Medications): This is a critical category
