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Inject the bacteriostatic water slowly down the inside wall of the vial, allowing the liquid to trickle down and dissolve the peptide cake indirectly
To ensure optimal FcRn binding, cellular transport, and PK properties of albumin-based biologics, we attempted to substitute the C-terminal L585 of albumin with an amino acid residue that would not be recognized by CPA
This mechanism may be attributed to the interference with copper ion transport leading to increased ROS levels, mitochondrial dysfunction and reduced ATP production, ultimately inhibiting Cu/Zn SOD1 activity (136)
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